| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 41 | Meiothermus ruber | AGCCTGGTTTTGTGGGCA | TGCTTGTTGCCCATCACCA | 59.72 | 60.15 | 265 | 117 |
100.00%
|
100.00%
|
| Primer 42 | Meiothermus ruber | AGCCTGGTTTTGTGGGCA | TTTGAAGGGCTGGTGTCCG | 59.72 | 60.23 | 215 | 117 |
100.00%
|
100.00%
|
| Primer 43 | Meiothermus ruber | TGATTGAGCAGGGCAGAGC | CGTATGCGCACGGCTAAAC | 60.08 | 60.01 | 200 | 115 |
100.00%
|
100.00%
|
| Primer 44 | Meiothermus ruber | AACCCTGCCTGCTCATCAC | ATAGCGCATGGATGGCCTC | 60.00 | 60.00 | 249 | 115 |
100.00%
|
100.00%
|
| Primer 45 | Meiothermus ruber | TGCAGTTGGACGCCTTGA | AGACCAGCGCTTCCTTGTC | 59.49 | 60.00 | 204 | 114 |
100.00%
|
100.00%
|
| Primer 46 | Meiothermus ruber | TCGCGTTCACGAAGCCAT | ACCCAGGTGTGCGAAAGAC | 60.05 | 60.23 | 261 | 114 |
100.00%
|
100.00%
|
| Primer 47 | Meiothermus ruber | TGCGAGCCAAAATGCTGG | ATCATGCCCAGCACCTCAC | 59.04 | 60.08 | 241 | 114 |
100.00%
|
100.00%
|
| Primer 48 | Meiothermus ruber | AGCTGTCAGGCCTTTGCA | TTGCGGAACCACAGCTCA | 59.48 | 59.49 | 236 | 114 |
100.00%
|
100.00%
|
| Primer 49 | Meiothermus ruber | CGGGTTTGGGCTGTTCTGA | TTTCCATCGAGCAGCGCA | 60.23 | 60.05 | 250 | 114 |
100.00%
|
100.00%
|
| Primer 50 | Meiothermus ruber | ACGCTTCGGACGGATTGA | TTGCCTCGCAAACCCGAT | 59.03 | 59.97 | 210 | 114 |
100.00%
|
100.00%
|